%0 Journal Article %J Proceedings of the National Academy of Sciences of the United States of America %D 2001 %T Functionalized xenon as a biosensor %A Spence, M. M. %A Rubin, S. M. %A Dimitrov, I. E. %A Ruiz, E. J. %A Wemmer, D. E. %A Pines, A. %A Yao, S. Q. %A Tian, F. %A Schultz, P. G. %K mri %X

The detection of biological molecules and their interactions is a significant component of modern biomedical research. In current biosensor technologies, simultaneous detection is limited to a small number of analytes by the spectral overlap of their signals. We have developed an NMR-based xenon biosensor that capitalizes on the enhanced signal-to-noise, spectral simplicity, and chemical-shift sensitivity of laser-polarized xenon to detect specific biomolecules at the level of tens of nanomoles. We present results using xenon "functionalized" by a biotin-modified supramolecular cage to detect biotin-avidin binding. This biosensor methodology can be extended to a multiplexing assay for multiple analytes.

%B Proceedings of the National Academy of Sciences of the United States of America %V 98 %P 10654-10657 %8 Sep 11 %@ 0027-8424 %G English %U ://WOS:000170966800030 %N 19 %M WOS:000170966800030 %! Functionalized xenon as a biosensor %R Doi 10.1073/Pnas.191368398