%0 Journal Article %J Analytical Chemistry %D 2011 %T Remotely Detected NMR for the Characterization of Flow and Fast Chromatographic Separations Using Organic Polymer Monoliths %A Teisseyre, T. Z. %A Urban, J. %A Halpern-Manners, N. W. %A Chambers, S. D. %A Bajaj, V. S. %A Svec, F. %A Pines, A. %K visualization %X

An application of remotely detected magnetic resonance imaging is demonstrated for the characterization of flow and the detection of fast, small molecule separations within hypercrosslinked polymer monoliths. The hyper-cross-linked monoliths exhibited excellent ruggedness, with a transit time relative standard deviation of less than 2.1%, even after more than 300 column volumes were pumped through at high pressure and flow. Magnetic resonance imaging enabled high. resolution intensity and velocity-encoded images of mobile phase flow through the monolith. The images confirm that the presence of a polymer monolith within the capillary disrupts the parabolic laminar flow profile that is characteristic of mobile phase flow within an open tube. As a result, the mobile phase and analytes are equally distributed in the radial direction throughout the monolith. Also, in-line monitoring of chromatographic separations of small molecules at high flow rates is shown. The coupling of monolithic chromatography columns and NMR provides both real-time peak detection and chemical shift information for small aromatic molecules. These experiments demonstrate the unique power of magnetic resonance, both direct and remote, in studying chromatographic processes.

%B Analytical Chemistry %V 83 %P 6004-6010 %8 Aug 1 %@ 0003-2700 %G English %U ://WOS:000293252500029 %N 15 %M WOS:000293252500029 %! Remotely Detected NMR for the Characterization of Flow and Fast Chromatographic Separations Using Organic Polymer Monoliths %R Doi 10.1021/Ac2010108