Functionalized xenon as a biosensor

TitleFunctionalized xenon as a biosensor
Publication TypeJournal Article
Year of Publication2001
AuthorsSpence M.M, Rubin S.M, Dimitrov I.E, Ruiz E.J, Wemmer D.E, Pines A, Yao S.Q, Tian F., Schultz P.G
JournalProceedings of the National Academy of Sciences of the United States of America
Date PublishedSep 11
ISBN Number0027-8424
Accession NumberWOS:000170966800030

The detection of biological molecules and their interactions is a significant component of modern biomedical research. In current biosensor technologies, simultaneous detection is limited to a small number of analytes by the spectral overlap of their signals. We have developed an NMR-based xenon biosensor that capitalizes on the enhanced signal-to-noise, spectral simplicity, and chemical-shift sensitivity of laser-polarized xenon to detect specific biomolecules at the level of tens of nanomoles. We present results using xenon "functionalized" by a biotin-modified supramolecular cage to detect biotin-avidin binding. This biosensor methodology can be extended to a multiplexing assay for multiple analytes.

URL<Go to ISI>://WOS:000170966800030
Short TitleFunctionalized xenon as a biosensor
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