Temperature response of (129)Xe depolarization transfer and its application for ultrasensitive NMR detection

TitleTemperature response of (129)Xe depolarization transfer and its application for ultrasensitive NMR detection
Publication TypeJournal Article
Year of Publication2008
AuthorsSchroeder L., Meldrum T., Smith M., Lowery T.J, Wemmer D.E, Pines A
JournalPhysical Review Letters
Volume100
Issue25
Date PublishedJun 27
ISBN Number0031-9007
Accession NumberWOS:000257230500066
Keywordsbiosensors
Abstract

Trapping xenon in functionalized cryptophane cages makes the sensitivity of hyperpolarized (HP) (129)Xe available for specific NMR detection of biomolecules. Here, we study the signal transfer onto a reservoir of unbound HP xenon by gating the residence time of the nuclei in the cage through the temperature-dependant exchange rate. Temperature changes larger than similar to 0.6 K are detectable as an altered reservoir signal. The temperature response is adjustable with lower concentrations of caged xenon providing more sensitivity at higher temperatures. Ultrasensitive detection of functionalized cryptophane at 310 K is demonstrated with a concentration of 10 nM, corresponding to a similar to 4000-fold sensitivity enhancement compared to conventional detection. This makes HPNMR capable of detecting such constructs in concentrations far below the detection limit of benchtop uv-visible light absorbance.

URL<Go to ISI>://WOS:000257230500066
DOI10.1103/Physrevlett.100.257603
Short TitleTemperature response of (129)Xe depolarization transfer and its application for ultrasensitive NMR detection
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